The Automation Partnership – Automated NGS Library Preparation System

Horse IgM ELISA Kit
IHSIGMKT	Innovative research	each
Description: Horse IgM ELISA Kit
Horse Vitamin D(VD)
QY-E120083	Qayee Biotechnology	96T
Horse SAA ELISA kit
SAA-14	Life Diagnostics	1x 96 wells plate
Horse Cholinesterase Butyryl
IHSCHEB4KU	Innovative research	each
Description: Horse Cholinesterase Butyryl
Horse Cholinesterase Butyryl
IHSCHEB500UN	Innovative research	each
Description: Horse Cholinesterase Butyryl
Horse Red Blood Cells
88R-H002	Fitzgerald	20 ml
Description: Glutaraldehyde-stabilized freshly prepared Horse Red Blood Cells
Horse Red Blood Cells
ABC-TC3474	AcceGen	1 vial
Description: Glutaraldehyde stabilized red blood cells are produced from freshly washed horse RBCs by brief exposure to a glutaraldehyde-buffer solution and exhaustive washing in saline. This treatment largely preserves the native antigenicity of the erythrocytes

Accu-Tell COVID-19 IgG/IgM Rapid Test
GEN-B352-20tests	Accu test	20 tests	283.2 EUR
Accu-Tell COVID-19 IgG/IgM Rapid Test
GEN-B352-40tests	Accu test	40 tests	385.2 EUR
2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma)
GEN-402-25tests	All test	25 tests	292.8 EUR
Human TES(Testin) ELISA Kit
EH1738	FN Test	96T	681.12 EUR
T(Testosterone) ELISA Kit
EU0400	FN Test	96T	571.5 EUR
Rat T(Testosterone) ELISA Kit
ER1462	FN Test	96T	628.92 EUR
Recombinant human Testin
P2531	FN Test	100ug	Ask for price
Mouse Testosterone ELISA Kit
EM1850	FN Test	96T	628.92 EUR

Our used rec. in Pubmed.

Monoclonal MVD Antibody (monoclonal) (M01), Clone: 2A7
APR08578G	Leading Biology	0.1mg	580.8 EUR
Monoclonal NNT Antibody (monoclonal) (M01), Clone: 1D6
APR08763G	Leading Biology	0.1mg	580.8 EUR
Monoclonal SMS Antibody (monoclonal) (M01), Clone: 1G6
APR10186G	Leading Biology	0.1mg	580.8 EUR
Monoclonal SP1 Antibody (monoclonal) (M01), Clone: 4H6
APR10212G	Leading Biology	0.1mg	580.8 EUR
Monoclonal SP1 Antibody (monoclonal) (M02), Clone: 1A5
APR10213G	Leading Biology	0.1mg	580.8 EUR

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Compare antibodies lab reagents for research

December 29, 2022 Lieven Leave a comment

Suppliers for Lab reagents

Bovine Prorenin
E01A85845	BlueGene	96T
Description: ELISA
Bovine Neuritin
E01A84833	BlueGene	96T
Description: ELISA
Bovine Serum Albumin (BSA) Monoclonal Antibody (Bovine), FITC
4-MAA248Bo21-FITC	Cloud-Clone	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Bovine Bovine Serum Albumin (BSA). This antibody is labeled with FITC.
Bovine Infectious Bovine Rhinotracheitis Ab(IBR) ELISA Kit
QY-E60095	Qayee Biotechnology	96T
Bovine Aprotinin
7-04414	CHI Scientific	100mg
Bovine Aprotinin
7-04415	CHI Scientific	250mg
Bovine Aprotinin
7-04416	CHI Scientific	1gr

H2B Antibody Antibody
E11-184659	EnoGene	100ug/100ul	225 EUR
anti- Antibody^Polyclonal antibody control antibody
LSMab09882	Lifescience Market	100 ug	525.6 EUR
CD11b Antibody Antibody
ABD2911	Lifescience Market	100 ug	525.6 EUR
ASAP1 antibody Antibody
DF8746-100ul	Affinity Biosciences	100ul	280 EUR
ASAP1 antibody Antibody
DF8746-200ul	Affinity Biosciences	200ul	350 EUR
ASAP1 antibody Antibody
DF8746	Affinity Biosciences	100ul	280 EUR
ZNF98 Antibody Antibody
E36403	EnoGene	100μg	275 EUR
CD11b Antibody Antibody
E19-2911-1	EnoGene	50ug/50ul	145 EUR

Our used recombinants in Pubmed.

Anti-Anti-MARCH8 Antibody antibody
STJ114828	St John's Laboratory	100 µl	332.4 EUR
Anti-Anti-SEPT12 Antibody antibody
STJ117759	St John's Laboratory	100 µl	332.4 EUR
Anti-Anti-MARCH6 Antibody antibody
STJ118549	St John's Laboratory	100 µl	332.4 EUR
Anti-Anti-MARCH6 Antibody antibody
STJ118550	St John's Laboratory	100 µl	332.4 EUR
Anti-Anti-MARCH7 Antibody antibody
STJ118752	St John's Laboratory	100 µl	332.4 EUR

bought from

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Prediction of Loss of Muscle Mass in Sarcopenia Using Ultrasonic Diaphragm Excursion

February 13, 2022 Lieven Leave a comment

Background: The diagnosis of sarcopenia is based on the mass and function of appendicular skeletal muscle. It is not clear whether diaphragm excursion is related to muscle mass loss. We try to fill the gap by measuring ultrasonic diaphragm excursion during quiet breathing (Dq) and forced deep breathing (Df) and test whether they could predict the muscle mass loss in sarcopenia.

Methods: The subjects are recruited from the elderly patients diagnosed with pulmonary nodules in community physical examination. According to the definition, the subjects were divided into group A (who did not meet the diagnostic criteria for muscle mass loss in sarcopenia) and group B (who met the criteria). Participants were assessed for ultrasonic diaphragm excursion, pulmonary function, and cardiopulmonary exercise testing. Logistic regression was used to assess the correlation between right diaphragm excursion and skeletal muscle mass, and receiver-operating characteristic curve (ROC) was applied to determine the best threshold.

Results: We recruited 64 elderly participants: 52 in group A (39 males) and 12 in group B (8 males). The Df in group A were higher than in group B (6.02 (5.44-6.60) vs. 4.31 (3.53-5.09) cm, P=0.008). The difference also exists in FVC, FEV1.0, PEF, Pimax, WRmax, and VO₂max, but neither in Dq. Logical regression showed that Df was negatively related to muscle mass (B = -0.525, OR = 0.591 (0.378-0.926), P=0.022), even after adjusted age. Based on ROC, a cutoff value of 5.27 cm (AUC = 0.7783, P=0.0028) was selected, and Df ≤ 5.27 cm indicates the increase in odds https://biodas.org/ of existing muscle mass loss.

Conclusion: Ultrasonic diaphragm excursion in forced deep breath is helpful for predicting muscle mass loss in sarcopenia.

Application and Validation of the Tricuspid Annular Plane Systolic Excursion/Systolic Pulmonary Artery Pressure Ratio in Patients with Ischemic and Non-Ischemic Cardiomyopathy

The main aim of this study was to assess the prognostic utility of TAPSE/PASP as an echocardiographic parameter of maladaptive RV remodeling in cardiomyopathy patients using cardiac magnetic resonance (CMR) imaging. Furthermore, we sought to compare TAPSE/PASP to TAPSE. The association of the echocardiographic parameters TAPSE/PASP and TAPSE with CMR parameters of RV and LV remodeling was evaluated in 111 patients with ischemic and non-ischemic cardiomyopathy and cut-off values for maladaptive RV remodeling were defined.

In a second step, the prognostic value of TAPSE/PASP and its cut-off value were analyzed regarding mortality in a validation cohort consisting of 221 patients with ischemic and non-ischemic cardiomyopathy. A low TAPSE/PASP (<0.38 mm/mmHg) and TAPSE (<16 mm) were associated with a lower RVEF and a long-axis RV global longitudinal strain (GLS) as well as higher RVESVI, RVEDVI and NT-proBNP.

A low TAPSE/PASP, but not TAPSE, was associated with a lower LVEF and long-axis LV GLS, and a higher LVESVI, LVEDVI and T1 relaxation time at the interventricular septum and the RV insertion points. Furthermore, in the validation cohort, low TAPSE/PASP was associated with a higher mortality and TAPSE/PASP was an independent predictor of mortality. TAPSE/PASP is a predictor of maladaptive RV and LV remodeling associated with poor outcomes in cardiomyopathy patients.

Preoperative Computed Tomography Angiography Reveals Leaflet-Specific Calcification and Excursion Patterns in Aortic Stenosis

Background: Computed tomography-based evaluation of aortic stenosis (AS) by calcium scoring does not consider interleaflet differences in leaflet characteristics. Here, we sought to examine the functional implications of these differences.

Methods: We retrospectively reviewed the computed tomography angiograms of 200 male patients with degenerative calcific AS undergoing transcatheter aortic valve replacement and 20 male patients with normal aortic valves. We compared the computed tomography angiography (CTA)-derived aortic valve leaflet calcification load (AVLC_CTA), appearance, and systolic leaflet excursion (LE_sys) of individual leaflets. We performed computer simulations of normal valves to investigate how interleaflet differences in LE_sys affect aortic valve area. We used linear regression to identify predictors of leaflet-specific calcification in patients with AS.

Results: In patients with AS, the noncoronary cusp (NCC) carried the greatest AVLC_CTA (365.9 [237.3-595.4] Agatston unit), compared to the left coronary cusp (LCC, 278.5 [169.2-478.8] Agatston unit) and the right coronary cusp (RCC, 240.6 [137.3-439.0] Agatston unit; both P<0.001). However, LCC conferred the least LE_sys (42.8º [38.8º-49.0º]) compared to NCC (44.8º [41.1º-49.78º], P=0.001) and RCC (47.7º [42.0º-52.3º], P<0.001) and was more often characterized as predominantly thickened (23.5%) compared to NCC (12.5%) and RCC (16.5%). Computer simulations of normal valves revealed greater reductions in aortic valve area following closures of NCC (-32.2 [-38.4 to -25.8]%) and RCC (-35.7 [-40.2 to -32.9]%) than LCC (-24.5 [-28.5 to -18.3]%; both P<0.001). By linear regression, the AVLC_CTA of NCC and RCC, but not LCC, predicted LE_sys (both P<0.001) in patients with AS. Both ostial occlusion and ostial height of the right coronary artery predicted AVLC_{CTA, RCC} (P=0.005 and P=0.001).

Conclusions: In male patients, the AVLC_CTA of NCC and RCC contribute more to AS than that of LCC. LCC’s propensity for noncalcific leaflet thickening and worse LE_sys, however, should not be underestimated when using calcium scores to assess AS severity.

Keywords: aortic valve stenosis; calcium; computed tomography angiography; computer simulation; transcatheter aortic valve replacement.

Polysomnographic Plethysmography Excursions are Reduced in Obese Elderly Men

Sleep apnea is a widespread disorder and is defined by the complete or partial cessation of breathing. Obstructive sleep apnea (OSA) is caused by an obstruction in the upper airway while central sleep apnea (CSA) is characterized by a diminished or absent respiratory effort. It is crucial to differentiate between these respiratory subtypes as they require radically different treatments.

Currently, diagnostic polysomnography (PSG) is used to determine respiratory thoracic and abdominal movement patterns using plethysmography belt signals, to distinguish between OSA and CSA. There is significant manual technician interrater variability between these classifications, especially in the evaluation of CSA. We hypothesize that an increased body mass index (BMI) will cause decreased belt signal excursions that increase false scorings of CSA.

The hypothesis was investigated by calculating the envelope as a continuous signal of belt signals in 2833 subjects from the MrOS Sleep Study and extracting a mean value of each of the envelopes for each subject. Using linear regression, we found that an increased BMI was associated with lower excursions during REM sleep (-0.013 [mV] thoracic and -0.018 [mV] abdominal, per BMI) and non-REM (-0.014 [mV] thoracic and -0.012 [mV] abdominal, per BMI). We conclude that increased BMI leads to lower excursions in the belt signals during event-free sleep, and that OSA and CSA events are harder to distinguish in subjects with high BMI. This has a major implication for the correct identification of CSA/OSA and its treatment.

TRAC Antibody
1-CSB-PA024144LA01HU	Cusabio	380.40 EUR 402.00 EUR	100ug 50ug
TRAC Antibody
DF14496-100ul	Affinity Biosciences	100ul	280 EUR
TRAC Antibody
DF14496-200ul	Affinity Biosciences	200ul	350 EUR
TRAC Antibody
DF14496	Affinity Biosciences	100ul	280 EUR
TRAC Polyclonal Antibody
A55102	EpiGentek	684.66 EUR 117.70 EUR 302.50 EUR 423.50 EUR	100 µg 20 ul 50 ul 100 ul
TRAC-1 Polyclonal Antibody
ABP55686-003ml	Abbkine	0.03ml	189.6 EUR
TRAC-1 Polyclonal Antibody
ABP55686-01ml	Abbkine	0.1ml	346.8 EUR
TRAC-1 Polyclonal Antibody
ABP55686-02ml	Abbkine	0.2ml	496.8 EUR
TRAC-1 Polyclonal Antibody
ES6685-100ul	ELK Biotech	100ul	334.8 EUR
TRAC-1 Polyclonal Antibody
ES6685-50ul	ELK Biotech	50ul	248.4 EUR
TRAC-1 Polyclonal Antibody
E20-74715	EnoGene	100ug	225 EUR
TRAC Antibody / TCR alpha
RQ4617	NSJ Bioreagents	100ug	356.15 EUR
TRAC Antibody, HRP conjugated
1-CSB-PA024144LB01HU	Cusabio	380.40 EUR 402.00 EUR	100ug 50ug
TRAC Antibody, FITC conjugated
1-CSB-PA024144LC01HU	Cusabio	380.40 EUR 402.00 EUR	100ug 50ug
Recombinant Murine TRAC (CCL17)
MECCP-1701	Cyagen	5ug	Ask for price
TRAC Antibody, Biotin conjugated
1-CSB-PA024144LD01HU	Cusabio	380.40 EUR 402.00 EUR	100ug 50ug
TRAC ORF Vector (Human) (pORF)
ORF034213	ABM	1.0 ug DNA	Ask for price
Human TRAC Protein, His Tag
TRC-H52H3	ACROBIOSYSTEMS	100ug	2493.1 EUR
Anti-TCR alpha/TRAC Antibody
A05315	BosterBio	100ug/vial	400.8 EUR

Centrifuge-free dispersive liquid-liquid microextraction coupled with thin-film microextraction for the preconcentration of molinate in real samples by ion mobility spectrometry

February 13, 2022 Lieven Leave a comment

A tandem microextraction method, centrifuge free dispersive liquid-liquid microextraction and thin-film microextraction (DLLME-TFME), was used for analyzing molinate in environmental samples by ion mobility spectrometry (IMS). Considering the IMS as a competitive detection system, coupling these two popular sample preparation methods reduces the effect of solvent interference and improves the sensitivity of the technique.

Trichloromethane and methanol were used as the extraction, and dispersive solvents for the DLLME method and electrospun polyacrylonitrile/copper-benzene-1,4-dicarboxylic acid fibers were used as a sorbent in the TFME method. Some effective experimental variables influencing the extraction efficiency of an analyte such as type and volume of dispersive and extraction solvents, solution pH, ionic strength, sonication time, and extraction time were studied.

The linear dynamic range of 0.5-50 μg L^-1 and the limit of detection of 0.1 μg L^-1 were obtained under optimized conditions. The relative standard deviations for intra-and inter-day analysis https://biodas.org/ were calculated less than 10%. The present method was used for the determination of molinate in different real samples such as agricultural wastewater, well water, river water, and apple, and the recovery was obtained between 82% and 113%, for the spiked samples.

Acoustofluidic centrifuge for nanoparticle enrichment and separation

Liquid droplets have been studied for decades and have recently experienced renewed attention as a simplified model for numerous fascinating physical phenomena occurring on size scales from the cell nucleus to stellar black holes. Here, we present an acoustofluidic centrifugation technique that leverages an entanglement of acoustic wave actuation and the spin of a fluidic droplet to enable nanoparticle enrichment and separation.

By combining acoustic streaming and droplet spinning, rapid (<1 min) nanoparticle concentration and size-based separation are achieved with a resolution sufficient to identify and isolate exosome subpopulations.

The underlying physical mechanisms have been characterized both numerically and experimentally, and the ability to process biological samples (including DNA segments and exosome subpopulations) has been successfully demonstrated. Together, this acoustofluidic centrifuge overcomes existing limitations in the manipulation of nanoscale (<100 nm) bioparticles and can be valuable for various applications in the fields of biology, chemistry, engineering, material science, and medicine.

Quantitative Evaluation of a Telerobotic System for Vascular Ultrasound Measurement on a Short Arm Human Centrifuge

Artificial Gravity generated by Short Arm Human Centrifuges is a promising multi-system countermeasure for physiological deconditioning during long duration space flights. To allow a continuous assessment of cardiovascular hemodynamics during centrifugation, a telerobotic robotic system holding an ultrasound probe has been installed on a Short Arm Human Centrifuge.
A feasibility study was conducted to define the use capabilities and limitations of such a novel method. The objective of this study is to estimate the reproducibility and precision of remotely controlled vascular ultrasound assessment under centrifugation by assessing peripheral vascular diameter and wall distension. Four repeated centrifugation runs of 5 min, with 2.4 g at feet level, were performed including a 15 min rest between each run for a group of eight healthy male volunteers. Vascular diameter and distention were assessed for the common carotid artery (CCA) and the femoral artery (FA) by ultrasound imaging using a 10 MHz linear array probe (Mylab1, Esaote).
Ultrasound measurements were consecutively performed: a) by an expert user in hand-held mode in standing as well as supine position, b) using the telerobotic arm without centrifugation as baseline and c) using the telerobotic arm during centrifugation. Vascular responses were compared between baseline and under centrifugation. Inter-, intra-registration and group variability have been assessed for hand-held and remotely controlled examination.
The results show that intra-registration variability, σ _h , was always smaller than inter-registration variability, σ _m, that is in turned smaller than the inter-subject variability σ _g (σ _h < σ _m < σ _g). Centrifugation caused no significant changes in CCA diameter but a lower carotid distension compared to manual and robotic ultrasound in supine position (p < 0.05). Femoral diameter was significantly decreased in hypergravity compared to robotic sonography without centrifugation.
A good reproducibility and precision of the remotely controlled vascular ultrasound assessment under centrifugation could be demonstrated. In conclusion, arterial wall dynamics can be precisely assessed for the CCA and femoral artery during centrifugation using a telerobotic ultrasound measurement system. Potential improvements to further enhance reproducibility and safety of the system are discussed.

Assessment of bridge natural frequency as an indicator of scour using centrifuge modelling

One of the most prevalent causes of bridge failure around the world is “scour”-the gradual erosion of soil around a bridge foundation due to fast-flowing water. A reliable technique for monitoring scour would help bridge engineers take timely countermeasures to safeguard against failure. Although vibration-based techniques for monitoring structural damage have had limited success, primarily due to insufficient sensitivity, these have tended to focus on the detection of local damage. High natural frequency sensitivity has recently been reported for scour damage.

Previous experiments to investigate this have been limited as a result of the cost of full-scale testing and the fact that scaled-down soil-structure models tested outside a centrifuge do not adequately simulate full-scale behaviour. This paper describes the development of what is believed to be the first-ever centrifuge-testing programme to establish the sensitivity of bridge natural frequency to scour. A 1/60 scale model of a two-span integral bridge with 15 m spans was tested at varying levels of scour. For the fundamental mode of vibration, these tests found up to a 40% variation in natural frequency for 30% loss of embedment.

Models of three other types of foundation, which represent a shallow pad foundation, a deep pile bent and a deep monopile, were also tested in the centrifuge at different scour levels. The shallow foundation model showed lower frequency sensitivity to scour than the deep foundation models. Another important finding is that the frequency sensitivity to “global scour” is slightly higher than the sensitivity to “local scour”, for all foundation types. The level of frequency sensitivity (3.1-44% per scour depth equivalent to 30% of embedment of scour) detected in this experiment demonstrates the potential for using natural frequency as an indicator of both local and global scour of bridges, particularly those with deep foundations.

Microliter ultrafast centrifuge platform for size-based particle and cell separation and extraction using novel omnidirectional spiral surface acoustic waves

Asymmetric surface acoustic waves have been shown useful in separating particles and cells in many microfluidics designs, mostly notably sessile microdroplets. However, no one has successfully extracted target particles or cells for later use from such samples. We present a novel omnidirectional spiral surface acoustic wave (OSSAW) design that exploits a new cut of lithium niobate, 152 Y-rotated, to rapidly rotate a microliter sessile drop to ∼10 g, producing efficient multi-size particle separation.

We further extract the separated particles for the first time, demonstrating the ability to target specific particles, for example, platelets from mouse blood for further integrated point-of-care diagnostics. Within ∼5 s of surface acoustic wave actuation, particles with diameter of 5 μm and 1 μm can be separated into two portions with a purity of 83% and 97%, respectively.

Red blood cells and platelets within mouse blood are further demonstrated to be separated with a purity of 93% and 84%, respectively. These advancements potentially provide an effective platform for whole blood separation and point-of-care diagnostics without need for micro or nanoscale fluidic enclosures.

Centrifuge 5910 Ri S-4x750
E5943000564	Scientific Laboratory Supplies	EACH	14476.8 EUR
Centrifuge 5920R no rotor
E5948000060	Scientific Laboratory Supplies	EACH	12007.2 EUR
12 Mini Centrifuge 100-240V (US plug)
BCM1746	Bio Basic	1 pcs, 1 UNIT	680.54 EUR
Bottle Centrifuge Pp 250 Ml
3141-0250	Scientific Laboratory Supplies	PK4	132 EUR
Bottle Centrifuge Pp 450 Ml
3141-0500	Scientific Laboratory Supplies	PK4	172.8 EUR
F-32x0.2 rotor for centrifuge 5420
E5495404007	Scientific Laboratory Supplies	EACH	546 EUR
Centrifuge 5920R no rotor- IVD Only
E5948000069	Scientific Laboratory Supplies	EACH	12601.2 EUR
SLS Micro Centrifuge 7000rpm
SLS1660	Scientific Laboratory Supplies	EACH	240 EUR
Centrifuge 5420 with rotor IVD ONLY
E5420000067	Scientific Laboratory Supplies	EACH	2392.8 EUR
Centrifuge 5420 without rotor
E5420000369	Scientific Laboratory Supplies	EACH	1842 EUR
FA-24x2 rotor for centrifuge 5420
E5495400001	Scientific Laboratory Supplies	EACH	690 EUR
Centrifuge 5920R 15/50mL package
E5948000365	Scientific Laboratory Supplies	EACH	14616 EUR
Centrifuge 5920R 15/50mL package
E5948000460	Scientific Laboratory Supplies	EACH	14618.4 EUR
Centrifuge 5920R 15/50mL package
E5948000560	Scientific Laboratory Supplies	EACH	14878.8 EUR
Bottle Centrifuge Sb 250Ml
3123-0250	Scientific Laboratory Supplies	PK4	110.4 EUR
MyFuge™ 12 Mini Centrifuge 100-240V (US plug)
C1012	Benchmark Scientific	1 each	448.5 EUR
Centrifuge 5420 with rotor FA-24x2
E5420000164	Scientific Laboratory Supplies	EACH	2109.6 EUR
Centrifuge 5427R + FA-45-12-17 Rotor
E5409000660	Scientific Laboratory Supplies	EACH	6828 EUR
Centrifuge 5420 without rotor IVD ONLY
E5420000261	Scientific Laboratory Supplies	EACH	1964.4 EUR

Droplet Magnetofluidic Assay Platform for Quantitative Methylation-Specific PCR

February 13, 2022 Lieven Leave a comment

Early cancer detection requires identification of cellular changes resulting from oncogenesis. Abnormal DNA methylation patterns occurring early in tumor development have been widely identified as early biomarkers for multiple types of cancer tumors. Methylation-Specific PCR (MSP) has permitted highly sensitive detection of these methylation changes at known biomarker locations.

MSP requires multiple sample preparation steps including protein digestion, DNA isolation, and bisulfite conversion prior to detection. In this work, we present a streamlined assay platform and instrumentation for integration of all sample processing steps required to obtain quantitative MSP signal from raw biological samples through the use of droplet magnetofluidic principles. In conjunction with this platform, we present a streamlined protocol for solid-phase https://biodas.org/ DNA extraction from cells and bisulfite conversion of genomic DNA, minimizing the processing steps and reagent volume for implementation on a compact assay platform.

Sensory analysis of hepatitis B virus DNA for medicinal clinical diagnostics based on molybdenum doped ZnO nanowires field effect transistor biosensor; a comparative study to PCR test results

In this paper, a bio-sensing setup for investigating hepatitis B virus deoxyribonucleic acid (HBV DNA) diagnosis including rapid testing and field effect transistor (FET) in label free assay is proposed. The FET biosensor was fabricated by molybdenum doped ZnO nanowires (NWs) in easy method and cost-free approach. The materialized NWs were synthesized by physical vapor deposition (PVD) growth mechanism.

The molybdenum dopant could bring about vacancy sites for DNA adsorption and electric charge transfer. The capability of the fabricated biosensor was evaluated by investigating the PCR-verified samples known as True Positive (TP), True Negative (TN), False Positive (FP) and False Negative (FN). The FET biosensor could materialize the clinical tests on samples TP, TN, FP and FN and could distinguish the clinical samples from each other. The designed biosensor showed more precision than the PCR-outcomes by exhibiting more sensitivity on labeled samples known as FN.

This research has analytical and comparative study on fabricated biosensor performance. The achieved results show that the biosensor had significant response to samples which have not been carefully detected by PCR test. The fabricated biosensor showed high accuracy, precision, sensitivity, specificity and reproducibility for differentiating (TP), (TN), (FP) and (FN) samples from healthy and normal sample. The response sensitivity was calculated and biosensor showed a detection limit (LOD) of 1 pM. The biosensor demonstrated high response and satisfied signal in the concentration ranges from 1 pM to 10 μM.

ACE2-based capacitance sensor for rapid native SARS-CoV-2 detection in biological fluids and its correlation with real-time PCR

The spread of the SARS-CoV-2 and its increasing threat to human health worldwide have necessitated the development of new technological tools to combat the virus. Particular emphasis is given to the development of diagnostic methods that monitor the spread of the virus rapidly and effectively.
In this study, we report the development and testing of an antibody-free biosensor, based on the immobilization of ACE2 protein on the surface of gold interdigitated electrode. When the sensor was used in laboratory conditions for targeting the virus’ structural spike protein, it showed a limit of detection [LOD] of 750 pg/μL/mm². Thereafter, the response of the sensor to swab and saliva samples from hospitalized patients was examined.
The virus presence in the samples was confirmed by electrical effective capacitance measurements executed on the biosensor, and correlated with real-time PCR results. We verified that the biosensor can distinguish samples that are positive for the virus from those that are negative in a total of 7 positive and 16 negative samples.
In addition, the biosensor can be used for semi-quantitative measurement, since its measurements are divided into 3 areas, the negative samples, the weakly positive and the positive samples. Reproducibility of the experiments was demonstrated with at least 3 replicates and stability was tested by keeping the sensor standby for 7 days at 4 °C before repeating the experiment. This work presents a biosensor that can be used as a fast-screening test at point of care detection of SARS-CoV-2 since it needs less than 2 min to provide results and is of simple operation.

High-resolution melting PCR analysis for genotyping the gene polymorphism of TNF-α, TGF-β1, IL-10, and IFN-γ in lung transplant recipients

Background: High-resolution melting (HRM) analysis is a genotyping method which has the advantages of simple, rapid, low-cost and closed-tube operation.

Objectives: This study evaluated HRM analysis as an option for detecting the single nucleotide polymorphism (SNP) of cytokine, and profiled the distribution of cytokine gene polymorphism in the lung transplant recipients (LTRs).

Material and methods: High-resolution melting-polymerase chain reaction (HRM-PCR) assays for genotyping tumor necrosis factor alpha (TNF-α) (-308 A/G), tumor growth factor beta 1 (TGF-β1) (+869 T/C), interleukin 10 (IL-10) (-592 C/A, -819 T/C, -1082 G/A), and interferon gamma (IFN-γ) (+874 T/A) SNPs were developed on the LightCycler® 480. The SNPs of the aforementioned cytokine genes in 322 LTRs and 266 normal controls were detected using HRM-PCR approach. To confirm the accuracy of the HRM-PCR assay, we randomly selected 100 samples from the LTRs and detected the aforementioned SNPs with sequence-specific primer-polymerase chain reaction (SSP-PCR) method, using a commercial kit.

Results: The data show that the HRM-PCR assay can distinguish all the cytokine SNPs, and the results of HRM-PCR analysis are in complete concordance to the genotyping results obtained using a commercial kit (κ = 1.0). Our data also show that the allele and genotype frequencies of the abovementioned cytokine are not significantly different between the LTRs and the control groups (p > 0.05). In addition, we found the genotypes of TGF-β1 +869 associated with high expression phenotype were prevalent in the LTRs. On the contrary, for TNF-α -308, IL-10 and IFN-γ, the genotypes associated with low expression phenotype were most common in the LTRs.

Conclusions: In this study, we described a rapid, low-cost and high-throughput HRM-PCR technology for genotyping cytokine SNPs. Our data may be utilized for future studies examining the associations of cytokine gene polymorphisms with the prognosis of the LTRs.

A Real-Time PCR Assay for Detection of Low Pneumocystis jirovecii Levels

Here we report a new real-time PCR assay using SYBR Green which provides higher sensitivity for the specific detection of low levels of Pneumocystis jirovecii. To do so, two primer sets were designed, targeting the family of genes that code for the most abundant surface protein of Pneumocystis spp., namely the major surface glycoproteins (Msg), and the mitochondrial large subunit rRNA (mtLSUrRNA) multicopy gene, simultaneously detecting two regions. PCR methods are instrumental in detecting these low levels; however, current nested-PCR methods are time-consuming and complex.

To validate our new real-time Msg-A/mtLSUrRNA PCR protocol, we compared it with nested-PCR based on the detection of Pneumocystis mitochondrial large subunit rRNA (mtLSUrRNA), one of the main targets used to detect this pathogen. All samples identified as positive by the nested-PCR method were found positive using our new real-time PCR protocol, which also detected P. jirovecii in three nasal aspirate samples that were negative for both rounds of nested-PCR.

Furthermore, we read both rounds of the nested-PCR results for comparison and found that some samples with no PCR amplification, or with a feeble band in the first round, correlated with higher Ct values in our real-time Msg-A/mtLSUrRNA PCR. This finding demonstrates the ability of this new single-round protocol to detect low Pneumocystis levels. This new assay provides a valuable alternative for P. jirovecii detection, as it is both rapid and sensitive.

50UL UNIVERSAL FIT FILTER TIPS
TF-50	CORNING	1000/pk	501.6 EUR
100UL UNIVERSAL FIT FILTER TIPS
TF-100	CORNING	1000/pk	501.6 EUR
1000UL UNIVERSAL FIT FILTER TIPS
TF-1000	CORNING	1000/pk	278.4 EUR
200UL UNIVERSAL FIT FILTER TIPS
TF-200	CORNING	1000/pk	501.6 EUR
300UL UNIVERSAL FIT FILTER TIPS
TF-350	CORNING	1000/pk	501.6 EUR
20UL FILTER TIPS FOR P-20
TF-20	CORNING	1000/pk	501.6 EUR
100UL "MAXYMUM RECOVERY" FILTER TIPS
TF-100-L	CORNING	1000/pk	547.2 EUR
BIOHIT(R) FILTER PIPETTE TIPS 50-1200
Z757829-960EA	Scientific Laboratory Supplies	PK960	258.51 EUR
BRAND(R) FILTER TIPS RACKED TIPBOX 0
Z740051-960EA	Scientific Laboratory Supplies	PK960	154.8 EUR
300ul Uni Fit Rckd+Strl Filter Tips
PIP0144	Scientific Laboratory Supplies	PK4800	601.2 EUR
0.1-10UL FILTER TIPS FOR EPPENDORF ULTRAMICRO
TF-400	CORNING	1000/pk	501.6 EUR
0.5-10UL FILTER TIPS FOR P-2, ULTRAMICRO
TF-300	CORNING	1000/pk	501.6 EUR
TIPS,ISOTIP FILTER,100-1000UL,S,RK,100/1K
4809	CORNING	100/pk	141.6 EUR
TIPS,ISOTIP,FILTER,1-200UL,S,RK,96/960
4810	CORNING	96/pk	158.4 EUR
TIPS,ISOTIP FILTER,1-200UL,S,RK,96/960
4823	CORNING	96/pk	139.2 EUR
TIPS,ISOTIP,FILTER,0.2-10UL,S,RK,96/960
4807	CORNING	96/pk	141.6 EUR
TIPS,ISOTIP FILTER,0.5-10UL,S,RK,96/960
4808	CORNING	96/pk	189.6 EUR
100UL ZYMARK FILTER TIPS, RACKED & PRE-STERILIZED
ZTF-100-R-S	CORNING	960/pk	519.6 EUR
200UL ZYMARK FILTER TIPS, RACKED & PRE-STERILIZED
ZTF-200-R-S	CORNING	960/pk	519.6 EUR

Superhydrophobic paper in the development of disposable labware and lab-on-paper devices.

February 13, 2022 Lieven Leave a comment

Traditionally in superhydrophobic surfaces history, the focus has frequently settled on the use of complex processing methodologies using nonbiodegradable and costly materials. In light of recent events on lab-on-paper emergence, there are now some efforts for the production of superhydrophobic paper but still with little development and confined to the fabrication of flat devices.

This work gives a new look at the range of possible applications of bioinspired superhydrophobic paper-based substrates, obtained using a straightforward surface modification with poly(hydroxybutyrate). As an end-of-proof of the possibility to create lab-on-chip portable devices, the patterning of superhydrophobic paper with different wettable shapes is shown with low-cost approaches.

Furthermore, we suggest the use of superhydrophobic paper as an extremely low-cost material to design essential nonplanar lab apparatus, including reservoirs for liquid storage and manipulation, funnels, tips for pipettes, or accordion-shaped substrates for liquid transport or mixing. Such devices take the advantage of the self-cleaning and extremely water resistance properties of the surfaces as well as the actions that may be done with paper such as cut, glue, write, fold, warp, or burn.

The obtained substrates showed lower propensity to adsorb proteins than the original paper, kept superhydrophobic character upon ethylene oxide sterilization and are disposable, suggesting that the developing devices https://biodas.org/ could be especially adequate for use in contact with biological and hazardous materials.

Contaminating levels of zinc found in commonly-used labware and buffers affect glycine receptor currents.

Zinc is an allosteric modulator of glycine receptor function, enhancing the effects of glycine at nM to low μM concentrations, and inhibiting its effects at higher concentrations. Because of zinc’s high potency at the glycine receptor, there exists a possibility that effects attributed solely to exogenously-applied glycine in fact contain an undetected contribution of zinc acting as an allosteric modulator.

We found that glycine solutions made up in standard buffers and using deionized distilled water produced effects that could be decreased by the zinc chelator tricine. This phenomenon was observed in three different vials tested and persisted even if vials were extensively washed, suggesting the zinc was probably present in the buffer constituents. In addition, polystyrene, but not glass, pipets bore a contaminant that enhanced glycine receptor function and that could also be antagonized by tricine.

Our findings suggest that without checking for this effect using a chelator such as tricine, one cannot assume that responses elicited by glycine applied alone are not necessarily also partially due to some level of allosteric modulation by zinc.

Labware additives identified to be selective monoamine oxidase-B inhibitors

Plastic labware is used in all processes of modern pharmaceutical research, including compound storage and biological assays. The use of these plastics has created vast increases in productivity and cost savings as experiments moved from glass test tubes and capillary pipettes to plastic microplates and multichannel liquid handlers. One consequence of the use of plastic labware, however, is the potential release of contaminants and their resultant effects on biological assays.

We report herein the identification of biologically active substances released from a commonly used plastic microplate. The active contaminants were identified by gas chromatography-mass spectroscopy as dodecan-1-ol, dodecyl 3-(3-dodecoxy-3-oxopropyl)sulfanylpropanoate, and dodecanoic acid, and they were found to be selective monoamine oxidase-B inhibitors.

3D Printing in the Laboratory: Maximize Time and Funds with Customized and Open-Source Labware

3D printing, also known as additive manufacturing, is the computer-guided process of fabricating physical objects by depositing successive layers of material. It has transformed manufacturing across virtually every industry, bringing about incredible advances in research and medicine. The rapidly growing consumer market now includes convenient and affordable “desktop” 3D printers.
These are being used in the laboratory to create custom 3D-printed equipment, and a growing community of designers are contributing open-source, cost-effective innovations that can be used by both professionals and enthusiasts. User stories from investigators at the National Institutes of Health and the biomedical research community demonstrate the power of 3D printing to save valuable time and funding.
While adoption of 3D printing has been slow in the biosciences to date, the potential is vast. The market predicts that within several years, 3D printers could be commonplace within the home; with so many practical uses for 3D printing, we anticipate that the technology will also play an increasingly important role in the laboratory.

3D-Printed Labware for High-Throughput Immobilization of Enzymes

In continuous flow biocatalysis, chemical transformations can occur under milder, greener, more scalable, and safer conditions than conventional organic synthesis. However, the method typically involves extensive screening to optimize each enzyme’s immobilization on its solid support material. The task of weighing solids for large numbers of experiments poses a bottleneck for screening enzyme immobilization conditions. For example, screening conditions often require multiple replicates exploring different support chemistries, buffer compositions, and temperatures.

Thus, we report 3D-printed labware designed to measure and handle solids in multichannel format and expedite screening of enzyme immobilization conditions. To demonstrate the generality of these advances, alkaline phosphatase, glucose dehydrogenase, and laccase were screened for immobilization efficiency on seven resins. The results illustrate the requirements for optimization of each enzyme’s loading and resin choice for optimal catalytic performance. Here, 3D-printed labware can decrease the requirements for an experimentalist’s time by >95%. The approach to rapid optimization of enzyme immobilization is applicable to any enzyme and many solid support resins. Furthermore, the reported devices deliver precise and accurate aliquots of essentially any granular solid material.

Additive manufactured customizable labware for biotechnological purposes

Yet already developed in the 1980s, the rise of 3D printing technology did not start until the beginning of this millennium as important patents expired, which opened the technology to a whole new group of potential users. One of the first who used this manufacturing tool in biotechnology was Lücking et al. in 2012, demonstrating potential uses 1, 2. This study shows applications of custom-built 3D-printed parts for biotechnological experiments.
It gives an overview about the objects’ computer-aided design (CAD) followed by its manufacturing process and basic studies on the used printing material in terms of biocompatibility and manageability. Using the stereolithographic (SLA) 3D-printing technology, a customizable shake flask lid was developed, which was successfully used to perform a bacterial fed-batch shake flask cultivation. The lid provides Luer connectors and tube adapters, allowing both sampling and feeding without interrupting the process. In addition, the digital blueprint the lid is based on, is designed for a modular use and can be modified to fit specific needs.
All connectors can be changed and substituted in this CAD software-based file. Hence, the lid can be used for other applications, as well. The used printing material was tested for biocompatibility and showed no toxic effects neither on mammalian, nor on bacteria cells. Furthermore an SDS-PAGE-comb was drawn and printed and its usability evaluated to demonstrate the usefulness of 3D printing for everyday labware. The used manufacturing technique for the comb (multi jet printing, MJP) generates highly smooth surfaces, allowing this application.

Purite Labwater 1 Deioniser Cartridge
WAT3314	Scientific Laboratory Supplies	EACH	157.2 EUR
Purite Labwater 2 Deioniser Cartridge
WAT3316	Scientific Laboratory Supplies	EACH	235.2 EUR
Purite Labwater 1 Water Deioniser with Coil and Gun
WAT3002	Scientific Laboratory Supplies	EACH	739.2 EUR
Purite Labwater 2 Water Deioniser with Coil and Gun
WAT3006	Scientific Laboratory Supplies	EACH	912 EUR
LabWipes, Small (4.5"x8.3"), 280/box
S.26415	IHC World	-	4.8 EUR
Grant Labwise Software
BAT3820	Scientific Laboratory Supplies	EACH	138 EUR
Pinfed Slide Labels, 1200 labels/pack
MLPF6-1200	IHC World	-	14.5 EUR
4400 Lab Coat+Zip White Size L
SAF0245	Scientific Laboratory Supplies	PK50	332.93 EUR
4400 Lab Coat+Zip White Size M
SAF0246	Scientific Laboratory Supplies	PK50	338.15 EUR
4400 Lab Coat+Zip White Size S
SAF0247	Scientific Laboratory Supplies	PK50	359.04 EUR
4400 Lab Coat+Zip White Size XL
SAF0248	Scientific Laboratory Supplies	PK50	338.15 EUR
4400 Lab Coat+Zip White Size XXL
SAF0242	Scientific Laboratory Supplies	PK50	338.15 EUR
4400 Lab Coat+Zip White Size 4XL
SAF0244	Scientific Laboratory Supplies	PK50	353.82 EUR
GHS-CLP Pictogram Labels Various GHS Labels 120 Labels
LAB4592	Scientific Laboratory Supplies	EACH	14.6 EUR
Sartoclear Dynamics Lab V 1000mL 10 g
FIL1260	Scientific Laboratory Supplies	PK24	1034.4 EUR
Sartoclear Dynamics Lab V 1000mL 20 g
FIL1262	Scientific Laboratory Supplies	PK12	543.6 EUR
Sartoclear Dynamics Lab V 1000mL 40 g
FIL1264	Scientific Laboratory Supplies	PK12	604.8 EUR
Tygon Formula 2375 Laboratory Tubing
Z685615-1PAK	Scientific Laboratory Supplies	15MTR	176.4 EUR
Tygon Formula E 3603 Laboratory Tubing
Z765171-50FT	Scientific Laboratory Supplies	EACH	495.6 EUR

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Horse IgM ELISA Kit

Horse Vitamin D(VD)

Horse SAA ELISA kit

Horse Cholinesterase Butyryl

Horse Cholinesterase Butyryl

Horse Red Blood Cells

Horse Red Blood Cells

Accu-Tell COVID-19 IgG/IgM Rapid Test

Accu-Tell COVID-19 IgG/IgM Rapid Test

2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma)

Human TES(Testin) ELISA Kit

T(Testosterone) ELISA Kit

Rat T(Testosterone) ELISA Kit

Recombinant human Testin

Mouse Testosterone ELISA Kit

Monoclonal MVD Antibody (monoclonal) (M01), Clone: 2A7

Monoclonal NNT Antibody (monoclonal) (M01), Clone: 1D6

Monoclonal SMS Antibody (monoclonal) (M01), Clone: 1G6

Monoclonal SP1 Antibody (monoclonal) (M01), Clone: 4H6

Monoclonal SP1 Antibody (monoclonal) (M02), Clone: 1A5

Bovine Prorenin

Bovine Neuritin

Bovine Serum Albumin (BSA) Monoclonal Antibody (Bovine), FITC

Bovine Infectious Bovine Rhinotracheitis Ab(IBR) ELISA Kit

Bovine Aprotinin

Bovine Aprotinin

Bovine Aprotinin

H2B Antibody Antibody

anti- Antibody^Polyclonal antibody control antibody

CD11b Antibody Antibody

ASAP1 antibody Antibody

ASAP1 antibody Antibody

ASAP1 antibody Antibody

ZNF98 Antibody Antibody

CD11b Antibody Antibody

Anti-Anti-MARCH8 Antibody antibody

Anti-Anti-SEPT12 Antibody antibody

Anti-Anti-MARCH6 Antibody antibody

Anti-Anti-MARCH6 Antibody antibody

Anti-Anti-MARCH7 Antibody antibody

Application and Validation of the Tricuspid Annular Plane Systolic Excursion/Systolic Pulmonary Artery Pressure Ratio in Patients with Ischemic and Non-Ischemic Cardiomyopathy

Preoperative Computed Tomography Angiography Reveals Leaflet-Specific Calcification and Excursion Patterns in Aortic Stenosis

Polysomnographic Plethysmography Excursions are Reduced in Obese Elderly Men

TRAC Antibody

TRAC Antibody

TRAC Antibody

TRAC Antibody

TRAC Polyclonal Antibody

TRAC-1 Polyclonal Antibody

TRAC-1 Polyclonal Antibody

TRAC-1 Polyclonal Antibody

TRAC-1 Polyclonal Antibody

TRAC-1 Polyclonal Antibody

TRAC-1 Polyclonal Antibody

TRAC Antibody / TCR alpha

TRAC Antibody, HRP conjugated

TRAC Antibody, FITC conjugated

Recombinant Murine TRAC (CCL17)

TRAC Antibody, Biotin conjugated

TRAC ORF Vector (Human) (pORF)

Human TRAC Protein, His Tag

Anti-TCR alpha/TRAC Antibody

Quantitative Evaluation of a Telerobotic System for Vascular Ultrasound Measurement on a Short Arm Human Centrifuge

Assessment of bridge natural frequency as an indicator of scour using centrifuge modelling

Microliter ultrafast centrifuge platform for size-based particle and cell separation and extraction using novel omnidirectional spiral surface acoustic waves

Centrifuge 5910 Ri S-4x750

Centrifuge 5920R no rotor

12 Mini Centrifuge 100-240V (US plug)

Bottle Centrifuge Pp 250 Ml

Bottle Centrifuge Pp 450 Ml

F-32x0.2 rotor for centrifuge 5420

Centrifuge 5920R no rotor- IVD Only

SLS Micro Centrifuge 7000rpm

Centrifuge 5420 with rotor IVD ONLY

Centrifuge 5420 without rotor

FA-24x2 rotor for centrifuge 5420

Centrifuge 5920R 15/50mL package

Centrifuge 5920R 15/50mL package

Centrifuge 5920R 15/50mL package

Bottle Centrifuge Sb 250Ml