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There are
two main aspects to Piccolo,
the culture of recombinant proteins(1)
and the processing of these cultures to provide
a range of possible outputs from cells through to
purified protein(2).
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The diagram below illustrates
a typical process flow for both microbial and insect
cells. |
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| ¹The
culture system provides a range
of culture parameters at the level of individual
microbial or insect cell cultures. Variables that
can be evaluated on a single run include: |
- Pre-induction and expression temperatures
- Time of harvest
- Inducer concentration
- Timing of induction based on culture
density
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- Media and antibiotic types
- Multiple microbial host or insect cell
lines
- Multiple microbial expression constructs
or recombinant baculovirus
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| ²The
downstream processing system in
Piccolo is designed for the reproducible delivery
of a range of alternative outputs from culture volumes
of 10 to 60mls for E.coli and 12.5 to 75mls for
insect cells. |
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